2J9E
Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-09 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.400, 77.800, 82.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 1.620 |
R-factor | 0.144 |
Rwork | 0.142 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j9c |
RMSD bond length | 0.013 |
RMSD bond angle | 1.636 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.030 | 0.160 |
Number of reflections | 47704 | |
<I/σ(I)> | 27.8 | 8.6 |
Completeness [%] | 92.0 | 57.7 |
Redundancy | 9.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | pH 4.60 |