2J9C
Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 122.700, 122.700, 45.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.650 - 1.300 |
| R-factor | 0.145 |
| Rwork | 0.143 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnk |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.068 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.250 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.120 | 0.510 |
| Number of reflections | 121571 | |
| <I/σ(I)> | 9.71 | 2.33 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 6.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | pH 4.60 |
