2J8U
Large CDR3a loop alteration as a function of MHC mutation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.422, 83.893, 122.273 |
| Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
| Resolution | 122.170 - 2.880 |
| R-factor | 0.269 |
| Rwork | 0.268 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lp9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 38683 | |
| <I/σ(I)> | 7 | 1.3 |
| Completeness [%] | 94.8 | 69 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.0 M NACL 20% PEG 8000 MES, PH 6.5 |
