2J7W
Dengue virus NS5 RNA dependent RNA polymerase domain complexed with 3' dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-24 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 163.716, 180.918, 58.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j7u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.010 | 0.630 |
| Number of reflections | 26929 | |
| <I/σ(I)> | 13.98 | 2.54 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS HCL AT PH 8.5, 0.8M K/NA TARTRATE, 0.5% W/V PEG MME 5K |
