2J7V
Structure of PBP-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.790, 91.900, 147.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.900 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PRE-ALIGNED STRUCTURES WITH PDB ENTRIES 1MF0 1e25 1bsg 1erm 1i2s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 94191 | |
| <I/σ(I)> | 13.9 | 3.8 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP. PROTEIN 10 MG/ML. RESERVOIR 500 UL HEPES 0.1 M PH 7.5, PROLINE 0.2 M, PEG-3350 10% (W/V) AND NAN3 0.02% (W/V). DROP 1UL PROTEIN AND 1 UL RESERVOIR |
