2J7P
GMPPNP-stabilized NG domain complex of the SRP GTPases Ffh and FtsY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.029, 129.556, 88.964 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.970 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1okk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 79821 | |
| <I/σ(I)> | 17.1 | 2.8 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M KI, 20% PEG 3350, pH 7.50 |
