2J7A
Crystal structure of cytochrome c nitrite reductase NrfHA complex from Desulfovibrio vulgaris
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEACH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.664, 258.117, 580.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 288.670 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.463 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.310 |
| Number of reflections | 443656 | |
| <I/σ(I)> | 8.3 | 3.1 |
| Completeness [%] | 83.9 | 67.3 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 10% PEG 4K, 0.1 M HEPES PH 7.5, 30 MM GLYCYL-GLYCYL-GLYCINE |
