2J6P
STRUCTURE OF AS-SB REDUCTASE FROM LEISHMANIA MAJOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.824, 111.824, 177.384 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.370 - 2.150 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24100 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.047 |
| RMSD bond angle | 2.881 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE-RESOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.410 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.160 |
| Number of reflections | 68637 | |
| <I/σ(I)> | 18.5 | 7.3 |
| Completeness [%] | 97.4 | 93.2 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.0 M (NH4)SO4, 100 MM HEPES PH 8.0, 15 MM NA2S2O3 |
