2J67
The TIR domain of human Toll-Like Receptor 10 (TLR10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.200, 43.300, 71.300 |
| Unit cell angles | 90.00, 101.00, 90.00 |
Refinement procedure
| Resolution | 23.430 - 2.200 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fyv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.524 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 19993 | |
| <I/σ(I)> | 13.8 | 3.5 |
| Completeness [%] | 97.6 | 92 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M NASCN, 11% PEG 3350 |
