2J62

Structure of a bacterial O-glcnacase in complex with glcnacstatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	I 21 21 21
Unit cell lengths	130.254, 145.920, 152.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.260
R-factor	0.18
Rwork	0.179
R-free	0.21900
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	1.401
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.250
Rmerge	0.070
Number of reflections	67921
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1