2J49

Crystal structure of yeast TAF5 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-07
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	91.340, 91.340, 55.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.640 - 2.300
R-factor	0.221
Rwork	0.215
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.157
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.040	0.200
Number of reflections	7594
<I/σ(I)>	42	4.7
Completeness [%]	99.0	99
Redundancy	5.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.05 M CACODYLATE PH 6.5,2.4 M NA FORMATE