2J41
Crystal structure of Staphylococcus aureus guanylate monophosphate kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-06 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.012, 93.986, 83.936 |
Unit cell angles | 90.00, 110.59, 90.00 |
Refinement procedure
Resolution | 29.400 - 1.900 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2anb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.322 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MrBUMP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.040 | 0.280 |
Number of reflections | 75428 | |
<I/σ(I)> | 31.6 | 2.96 |
Completeness [%] | 99.3 | 95.6 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.8 | 20% PEG 3350, 0.2 M LISO4, 0.1 M TRIS/HCL PH 8.8 |