2J25
Partially deglycosylated glucoceramidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-04 |
| Detector | ADSC CORPORATION |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.626, 280.821, 91.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.900 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OGS MOLECULE A |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.670 | 2.900 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.270 |
| Number of reflections | 30978 | |
| <I/σ(I)> | 13.75 | 5.3 |
| Completeness [%] | 98.7 | 98.6 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1M BIS-TRIS PH 5.5, 2M (NH4)2SO4 |
