2J1X

Human p53 core domain mutant M133L-V203A-Y220C-N239Y-N268D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	64.501, 71.109, 104.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.800 - 1.650
R-factor	0.185
Rwork	0.185
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uol
RMSD bond length	0.008
RMSD bond angle	1.500
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.800	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.060	0.190
Number of reflections	55177
<I/σ(I)>	24.4	8.2
Completeness [%]	94.2	89.2
Redundancy	8.1	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1