2J1W

Human p53 core domain mutant M133L-V143A-V203A-N239Y-N268D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	64.656, 71.066, 105.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 1.800
R-factor	0.185
Rwork	0.185
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uol
RMSD bond length	0.008
RMSD bond angle	1.500
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.400	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.290
Number of reflections	43176
<I/σ(I)>	21.2	5.7
Completeness [%]	95.2	90.8
Redundancy	6.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1