2J1L
Crystal Structure of Human Rho-related GTP-binding protein RhoD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.811, 66.810, 103.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mh1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.401 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.600 | 2.620 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 9805 | |
| <I/σ(I)> | 16.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 19% PEG6K, 0.4M NH4CL, 15% ETG, 0.05M MES PH6.0, pH 6.00 |
