2J0S

The crystal structure of the Exon Junction Complex at 2.2 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-17
Detector	MARRESEARCH
Spacegroup name	P 64
Unit cell lengths	169.440, 169.440, 71.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.730 - 2.210
R-factor	0.187
Rwork	0.185
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fuu 1fuk 1p27
RMSD bond length	0.014
RMSD bond angle	1.493
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.540
Number of reflections	58631
<I/σ(I)>	11.1	2.5
Completeness [%]	98.9	95.6
Redundancy	3.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				8% PEG 6000, 100 MM MGCL2, 100 MM MES PH 6.0