2J0M
Crystal structure a two-chain complex between the FERM and kinase domains of focal adhesion kinase.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-26 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.490, 90.600, 242.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.390 - 2.800 |
R-factor | 0.224 |
Rwork | 0.221 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1MP8 AND 2AEH |
RMSD bond length | 0.012 |
RMSD bond angle | 1.304 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.550 |
Number of reflections | 17515 | |
<I/σ(I)> | 11.5 | 2.73 |
Completeness [%] | 93.3 | 94.9 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 16% PEG20K, 250MM NACL, 100MM TRIS PH8.5, 10MM TCEP, pH 8.50 |