2J0L
Crystal structure of a the active conformation of the kinase domain of focal adhesion kinase with a phosphorylated activation loop.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-17 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.312, 71.809, 86.939 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.480 - 2.300 |
R-factor | 0.217 |
Rwork | 0.214 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mp8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.265 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.320 |
Number of reflections | 11899 | |
<I/σ(I)> | 12.1 | 2.51 |
Completeness [%] | 92.3 | 56.1 |
Redundancy | 4.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 26% PEG4K, 0.2M LISO4, 0.1M TRIS PH8.5, 10MM TCEP, pH 8.50 |