2J08
Thermus DNA photolyase with 8-Iod-riboflavin antenna chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 112.620, 112.620, 140.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.320 - 2.610 |
| R-factor | 0.167 |
| Rwork | 0.163 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iqr |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.281 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.400 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.140 |
| Number of reflections | 19019 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 91.5 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 50 MM TRIS-HCL, PH 8.5, 1.0 M NH4H2PO4, 11.8 MG/ML PROTEIN. CRYSTAL WAS SOAKED WITH 0.1 MM FMN FOR 90 MIN PRIOR TO FREEZING. |
