2J07
Thermus DNA photolyase with 8-HDF antenna chromophore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-04 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.620, 112.620, 140.262 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.860 - 1.950 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iqr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.462 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 18.900 |
High resolution limit [Å] | 1.950 |
Rmerge | 0.090 |
Number of reflections | 36800 |
<I/σ(I)> | 18.5 |
Completeness [%] | 99.2 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 50 MM TRIS-HCL PH 8.5, 1.0 M NH4 H2PO4, 11.8 MG/ML PROTEIN. CRYSTAL WAS SOAKED WITH 0.1 MM 8-HDF FOR 90 MIN PRIOR TO FREEZING. |