2IZR
Structure of casein kinase gamma 3 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.724, 56.724, 223.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.150 - 1.300 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2chl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.300 |
| Number of reflections | 99653 | |
| <I/σ(I)> | 22.4 | 3.8 |
| Completeness [%] | 95.9 | 79.7 |
| Redundancy | 6.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% PEG10K, 0.20 M MGCL2, 0.1 M TRIS PH=7.0, pH 7.00 |
