2IY4
X-ray structure of Dps from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.288, 172.708, 135.312 |
| Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.310 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.246 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 179045 | |
| <I/σ(I)> | 8.1 | 2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PEG 400 15-30 % W/V, HEPES 0.1 M PH=7.0, pH 7.00 |
