2IXF
Crystal structure of the ATPase domain of TAP1 with ATP (D645Q, Q678H mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-18 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.269, 108.917, 123.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.490 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ixe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 74956 | |
| <I/σ(I)> | 18.1 | 3.3 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP VAPOR DIFFUSION WITH THE RESERVOIR CONTAINING 0.75 M TRI-SODIUM CITRATE PH 8.0 . |
