2IX6
SHORT CHAIN SPECIFIC ACYL-COA OXIDASE FROM ARABIDOPSIS THALIANA, ACX4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 198.600, 198.600, 150.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 171.500 - 3.900 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ix5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.880 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.100 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.150 | 0.320 |
| Number of reflections | 27647 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 9.2 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M BIS-TRIS-PROPANE PH 7.0, 6% PEG8000, 8% GLYCEROL |
