2IX0
RNase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-07 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.836, 125.719, 66.244 |
Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
Resolution | 62.870 - 2.440 |
R-factor | 0.19 |
Rwork | 0.187 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ix1 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.320 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.940 | 2.530 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.080 | 0.420 |
Number of reflections | 31569 | |
<I/σ(I)> | 23.4 | 5.2 |
Completeness [%] | 98.9 | 98.2 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | VAPOUR DIFFUSION IN SITTING DROPS WITH 1.5 MICRO-L OF PROTEIN SOLUTION, 8-12 MG/ML 3% GLYCEROL 1 MM MGCL2 100 MM KCL AND 100 MM TRIS-HCL PH 8.0, AND 1.5 MICRO-L OF WELL SOLUTION, 22 % PEG 4K, 0.6M CA ACETATE AND 0.1M TRIS-HCL PH 8.5, AT 293 K |