2IWY
Human mitochondrial beta-ketoacyl ACP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.626, 96.061, 115.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.940 - 2.060 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.103 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 51373 | |
| <I/σ(I)> | 6.8 | 2.7 |
| Completeness [%] | 98.1 | 96.4 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 24% PEG-3350, 0.2 M NH4CL, pH 7.80 |
