2IUX
Human tACE mutant g1234
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.436, 85.149, 133.424 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2006 |
| Rwork | 0.201 |
| R-free | 0.23690 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.340 |
| Number of reflections | 13572 | |
| <I/σ(I)> | 7.7 | 2.8 |
| Completeness [%] | 83.6 | 80.4 |
| Redundancy | 39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.7 | 0.1M SODIUM ACETATE PH4.7 15% PEG 4000, 10UM ZINC SULPHATE, pH 4.70 |
