2IUI
Crystal structure of the PI3-kinase p85 N-terminal SH2 domain in complex with PDGFR phosphotyrosyl peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 278 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.760, 61.940, 108.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.400 |
R-factor | 0.1784 |
Rwork | 0.178 |
R-free | 0.24840 |
Structure solution method | OTHER |
RMSD bond length | 0.009 |
RMSD bond angle | 1.650 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.060 | 0.310 |
Number of reflections | 12930 | |
<I/σ(I)> | 23.37 | 4.92 |
Completeness [%] | 98.2 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M SODIUM ACETATE PH 4.6, 0.2M AMMONIUM ACETATE, 30% PEG4000 |