2IUH

Crystal structure of the PI3-kinase p85 N-terminal SH2 domain in complex with c-Kit phosphotyrosyl peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	278
Spacegroup name	P 41 21 2
Unit cell lengths	56.950, 56.950, 68.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.1539
Rwork	0.154
R-free	0.24500
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.728
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.140
Number of reflections	8651
<I/σ(I)>	30.87	10.84
Completeness [%]	96.5	92.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1M NA CITRATE PH5.6, 0.2M NA/K TARTRATE, 2M AMMONIUM SULFATE