2IUA
C. trachomatis LpxD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-01 |
| Detector | RIGAKU-MSC R-AXIS-4++ |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.679, 98.679, 284.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.250 - 2.700 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iu8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.510 |
| Number of reflections | 38280 | |
| <I/σ(I)> | 11.3 | 2 |
| Completeness [%] | 96.5 | 90.4 |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 1.2M AMMONIUM SULFATE, 0.1M MES, PH 6.5, 2% PEG 400, AND 1MM DTT. |
