2IU9
Chlamydia trachomatis LpxD with 100mM UDPGlcNAc (Complex II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-25 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.871, 98.872, 283.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.500 - 3.100 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iu8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.039 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 3.190 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.150 | 0.790 |
| Number of reflections | 26254 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN INCUBATED WITH 100MM UDPGLCNAC AND CRYSTALLIZED FROM 1.2M AMMONIUM SULFATE, 0.1M MES, PH 6.5, 2% DIOXANE, 1MM TCEP, AND 2MM UDPGLCNAC. |
