2ITN
Crystal structure of EGFR kinase domain G719S mutation in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-24 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 2 3 |
Unit cell lengths | 145.481, 145.481, 145.481 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.950 - 2.470 |
R-factor | 0.201 |
Rwork | 0.197 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m14 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.711 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.060 | 0.400 |
Number of reflections | 17802 | |
<I/σ(I)> | 40.4 | 6.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.7 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1.2M KNA TARTRATE, 0.1M HEPES 7.5 |