2ISO
Ternary complex of DNA Polymerase beta with a dideoxy terminated primer and 2'-deoxyguanosine 5'-beta, gamma-difluoromethylene triphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-12 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.700, 80.500, 55.400 |
| Unit cell angles | 90.00, 108.00, 90.00 |
Refinement procedure
| Resolution | 22.350 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fmp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.117 | 0.437 |
| Number of reflections | 22858 | |
| <I/σ(I)> | 8.7 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM Imidazole, pH 7.5, 350 mM Sodium Acetate, 16% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Imidazole | ||
| 2 | 1 | 1 | Sodium Acetate | ||
| 3 | 1 | 1 | PEG3350 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Imidazole | ||
| 6 | 1 | 2 | Sodium Acetate | ||
| 7 | 1 | 2 | PEG3350 | ||
| 8 | 1 | 2 | H2O |
