2IS9
Structure of yeast DCN-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 81.107, 81.107, 106.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.192 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.420 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.038 | 0.282 |
| Number of reflections | 26193 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M ammonium sulfate, 0.1M Tris HCL 8.5, 30% PEG MME 5000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
