2IS6
Crystal structure of UvrD-DNA-ADPMgF3 ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.336, 96.609, 110.930 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2IS4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.293 |
| Number of reflections | 102202 | |
| <I/σ(I)> | 30.1 | 2.67 |
| Completeness [%] | 93.5 | 63 |
| Redundancy | 5.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | PEG3350, Sodium Fluoride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium fluoride | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | sodium fluoride |
