2IRF
CRYSTAL STRUCTURE OF AN IRF-2/DNA COMPLEX.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | RIGAKU |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 132.300, 132.300, 296.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24300 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 18.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.200 | 2.100 |
| Rmerge | 0.062 * | |
| Total number of observations | 2494554 * | |
| Number of reflections | 69536 * | |
| Completeness [%] | 88.4 | 77.1 * |
| Redundancy | 35.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.8 | 4 * | 50 MM MES-K (PH 5.8), 50 MM KCL, 4% 2-MPD,, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MES | ||
| 5 | 1 | 2 | KCL | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Mes-K | 50 (mM) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | 4 (%) | ||
| 4 | 1 | drop | protein | ||
| 5 | 1 | drop | 13mer DNA |
