2IQ1
Crystal structure of human PPM1K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.442, 69.406, 77.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.557 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.674 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.147 | 0.848 |
| Total number of observations | 17878 | |
| Number of reflections | 15758 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 100mM Hepes pH 8, 18% PEG 10K, VAPOR DIFFUSION, temperature 294K |
