2IP4
Crystal Structure of Glycinamide Ribonucleotide Synthetase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9843 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.308, 98.068, 121.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.218 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gso |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | |
| Number of reflections | 29262 | |
| Completeness [%] | 95.6 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M ammonium sulfate, 0.1M MES, 30% PEGMME5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
