2IO2
Crystal structure of human Senp2 in complex with RanGAP1-SUMO-1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 64 2 2 |
| 格子定数 [Å] | 163.960, 163.960, 77.770 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 分解能 | 14.940 - 2.900 |
| Rwork | 0.268 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgz |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 3.000 |
| 分解能 [Å] (高) | 2.900 | 2.900 |
| Rmerge_l_obs | 0.071 | 0.292 |
| 独立反射数 | 13707 | |
| <I/σ(I)> | 25.8 | 2.5 |
| 完全性 [%] | 96.7 | 91.2 |
| 冗長性 | 13.5 | 3.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 12% PEG 4000, 0.1M Lithium chloride, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
