2ILU
Crystal structure of lactaldehyde dehydrogenase from E. coli: the binary complex with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 143.566, 143.566, 108.763 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
Rwork | 0.212 |
R-free | 0.25300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hg2 |
RMSD bond angle | 0.007 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.139 |
Number of reflections | 16757 |
<I/σ(I)> | 22.6 |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Bis-Tris or Tris, 2.0 M ammonium sulfate, pH pH 6.8 - 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |