2IJX
Crystal structure of PCNA3 monomer from Sulfolobus solfataricus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.773, 85.773, 264.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 1.900 |
| R-factor | 0.20493 |
| Rwork | 0.204 |
| R-free | 0.25055 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ud9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.102 | 0.491 |
| Number of reflections | 75947 | |
| <I/σ(I)> | 32.2 | 2.2 |
| Completeness [%] | 96.3 | 65.6 |
| Redundancy | 23.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12.5% PEG 3350, 0.15 M NH4Cl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
