2IJM
Crystal Structure of Focal Adhesion Kinase Domain with 2 molecules in the Asymmetric Unit Complexed with ADP and ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-01-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.432, 51.645, 66.544 |
| Unit cell angles | 99.81, 103.55, 90.69 |
Refinement procedure
| Resolution | 34.300 - 2.187 |
| R-factor | 0.21051 |
| Rwork | 0.207 |
| R-free | 0.27149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jba |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.365 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.270 |
| High resolution limit [Å] | 2.187 | 2.187 |
| Rmerge | 0.045 | 0.327 |
| Number of reflections | 28426 | |
| <I/σ(I)> | 16.3 | 4.1 |
| Completeness [%] | 95.1 | 70.9 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 | 30% (v/v) PEG-600, 10% glycerol, 0.1M HEPES pH 7.5, 0.05M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
