2IIM
SH3 Domain of Human Lck
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.95379 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.310, 50.310, 46.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| Rwork | 0.132 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SH3-Domain of 1LCK |
| RMSD bond length | 0.028 |
| RMSD bond angle | 0.065 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.052 | 0.180 |
| Number of reflections | 36787 | |
| <I/σ(I)> | 13.27 | 3.21 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 5.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1 uL of protein solution (protein concentration 15 mg/mL, 20 mM Tris-HCl (pH 7.5) buffer) and 1 uL reservoir solution containing 28% PEG 400, 0.1 M HEPES buffer (pH 7.5) and 0.2 M. no addtional cryoprotectant was needed, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
