2IHD
Crystal structure of Human Regulator of G-protein signaling 8, RGS8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 41 |
| Unit cell lengths | 65.967, 65.967, 76.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.500 - 1.700 |
| R-factor | 0.17707 |
| Rwork | 0.175 |
| R-free | 0.21119 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swissmodel based on pdb entry 2BT2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 17199 | |
| Completeness [%] | 95.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.20M NaI; 20.0% PEG 3350; 10.0% Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
