2IF0
Crystal Structure of mouse Rab27b bound to GDP in monoclinic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-31 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.960, 64.755, 153.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.100 - 2.800 |
| R-factor | 0.26163 |
| Rwork | 0.259 |
| R-free | 0.31492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iez |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.690 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.109 | 0.418 |
| Number of reflections | 14358 | |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 6.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 289 | MPD, Cacodylate, pH 5.5, VAPOR DIFFUSION, temperature 289K |
