2IDV
Crystal structure of wheat C113S mutant EIF4E bound TO 7-methyl-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.651, 58.227, 39.063 |
| Unit cell angles | 90.00, 115.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.200 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ej1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.063 | 0.147 |
| Number of reflections | 6939 | |
| <I/σ(I)> | 16.5 | 12.4 |
| Completeness [%] | 96.9 | 88.9 |
| Redundancy | 5.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 28% PEG4000, 0.1 M HEPES, 20 mM PHENOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
