2ID6
Crystal structure of transcriptional regulator (tm1030) at 1.75A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.27819 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 55.765, 66.012, 55.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.830 - 1.746 |
| R-factor | 0.21136 |
| Rwork | 0.209 |
| R-free | 0.25102 |
| Structure solution method | isomorphous replacement |
| Starting model (for MR) | 1z77 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.830 | 1.810 |
| High resolution limit [Å] | 1.746 | 1.746 |
| Rmerge | 0.062 | 0.397 |
| Number of reflections | 21339 | |
| <I/σ(I)> | 45.9 | 2.9 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 7.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 3%w/v Dextrose, 30%w/v PEG 2K MME, 0.1 M KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
