2ID5
Crystal Structure of the Lingo-1 Ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2005-10-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 201.517, 149.720, 157.486 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 158.110 - 2.698 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25500 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.301 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 158.110 | 2.800 |
High resolution limit [Å] | 2.698 | 2.698 |
Number of reflections | 122982 | |
<I/σ(I)> | 61 | 1.3 |
Completeness [%] | 98.9 | 94.7 |
Redundancy | 8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 1.4 M Am Sulfate, O.1 M Sodium Citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |