2IBZ
Yeast Cytochrome BC1 Complex with Stigmatellin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.473, 163.921, 147.276 |
| Unit cell angles | 90.00, 117.50, 90.00 |
Refinement procedure
| Resolution | 14.960 - 2.300 |
| Rwork | 0.222 |
| R-free | 0.25600 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.960 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.390 | |
| Number of reflections | 168517 | |
| <I/σ(I)> | 12.4 | 1.2 |
| Completeness [%] | 84.6 | 72.9 |
| Redundancy | 6.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | precipitant PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
